Pitfalls of glycated hemoglobin in the glycemic assessment of diabetes patients with hemoglobin louisville: role of serum fructosamine.

Fasting blood glucose measurements were made using the hexokinase method in a Cobas 6000 (Roche Diagnostics, Rotkreuz, Switzerland). Determinations of HbA1c were performed on ethylenediaminetetraacetic acid–anticoagulated blood sample by two methods: high-performance liquid chromatography (HPLC) (HA-8160 in diabetic mode, A. Menarini Diagnostics, Florence, Italy), which detected no abnormal hemoglobin (Hb); and immune-turbidimetric method (Tina-quant Hemoglobin A1c Gen3 in a Cobas 6000), which was used for two patients. Both methods were calibrated according to the International Federation of Clinical Chemistry and Laboratory Medicine (IFCC) Reference Measurement Procedure. Results are shown in Table 1.